|
KMID : 1150720180070020176
|
|
Integrative Medicine Research
2018 Volume.7 No. 2 p.176 ~ p.183
|
|
|
In vitro and in vivo antidiabetic effect of extracts of Melia azedarach, Zanthoxylum alatum, and Tanacetum nubigenum
|
|
Khan Mohammad Faheem
Rawat Arun Kumar
Khatoon Shahnaaz
Hussain Mohd Kamil
Mishra Arvind
Negi Devendra Singh
|
|
|
Abstract
|
|
|
Background: To investigate the antidiabetic effect of Himalayan Medicinal plants from India viz. Melia azedarach (Family: Meliaceae), Zanthoxylum alatum (Family: Rutaceae), Tanacetum nubigenum (Family: Asteraceae) using in-vitro as well as in-vivo approaches.
Methods: Their effects were examined on stimulation of glucose uptake by C2C12 cultured cell line, inhibitory effect on human recombinant Protein tyrosine phosphatase-1B (PTP-1B) and followed by the hypoglycaemic activity of extracts in Streptozotocin (STZ) induced diabetic rats.
Results: All prepared extracts had been found to enrich with polyphenolic, flavonoids, terpenoids, anthraquinones and saponins type of compounds. n-Butanol fraction of Zanthoxylum alatum showed maximum PTP-1B inhibition (61.9%) whereas ethanol extract of Tanacetum nubigenum showed strong stimulation of glucose uptake (+61.2%) in C2Cl2 myotubes. In STZ induced Sprague-Dawley rats, significant decrease in blood glucose level was observed in ethanol extract of Melia azaderach treated group as 14.8% (p < 0.01) whereas in the ethanol extract of Tanacetum nubigenum treated group, it was observed as 15.5% (p < 0.01) compare to metformin which showed 26.8% (p < 0.01) lowering of blood glucose in the same time duration of 5 h study.
Conclusion: This study demonstrated that these plants have a significant therapeutic value in type-2-diabetes mellitus and related complications thus supporting their traditional uses in Indian traditional system of medicine.
|
|
|
KEYWORD
|
|
|
Type-2 diabetes mellitus, C2C12 cultured cell line, STZ induced diabetic rats, PTP-1B
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|